Abstract: Tobacco intake associated with fitness dangers, morbidity and mortality have been growing. The prevalence of tobacco uses and nicotine dependence is very high among sanitary workers as compared to the general population. Nicotine dependence is one of the major public health problems in developing and developed countries. Very few studies were examined to assess nicotine dependence among housekeeping staff in hospitals. This study aimed to assess the nicotine dependence among housekeeping staff of AIIMS Bhubaneswar and to find the association between nicotine dependence and demographic characteristics such as age, gender, education, experience, duration of use, age of initiation and last use. A cross-sectional study was conducted to assess the nicotine dependence among the housekeeping staff of AIIMS Bhubaneswar. The information was collected using a demographic proforma, screening tool, Nicotine dependence tool- (a) Fagerstorm Test for Nicotine Dependence- (FTND) and (b) Fagerstorm Test for Nicotine Dependence -Smokeless Tobacco (FTND-ST) by self-reporting. Among 338 participants screened, 224 users showed nicotine dependence. Among 224 housekeeping staff, 58 users consume pan, 63 users use cigarettes and 103 consume both (Pan and Cigarette). Nicotine dependence is significantly associated with economic status and age of initiation and not significantly associated with demographic variables such as age, gender, education, marital status, religion, and job experience. Study concluded that Hospital-led control effort works required to make behavioural changes and prevention of tobacco use among housekeeping staff to save their lives, protect health and beat tobacco consumption to support National goal of ‘Nasha Mukt Bharat’.