Background and Aim: Obesity is the most prevalent metabolic disorder in industrialized nations. A common link between obesity and various other related disorders, including diabetes, insulin resistance, atherosclerosis, dyslipidemia, hypertension, inflammatory and cardiovascular disease. Fibronectin 1 (FN1) is a large extracellular glycoprotein located on the surface of animal cells and serves as the primary non-collagenous component of the extracellular matrix (ECM) and basement membrane. In the *present* -*study*, we aimed to assess serum levels of FN1 in obesity individuals and to – *evaluate* associations with the biochemical markers. Materials and Methods: The *study* was involving <120> Iraqi participants, comprising <60> obesity patients (<15>males / <45> females), their ages ranged from <25-55> years and <60>healthy control (<15>males / <45>females), their ages ranged from <25-55> years. Serum levels of FN1 and various demographic, anthropometric and biochemical parameters including age, gender, BMI; W/H; SBP; DBP; TNF-α; *T-CHO*; *HDL-C*; *TG*; *LDL-C*; *VLDL-C* and FSH were *measured* in all subjects. To compare the two groups and correlations evaluation among the investigated parameters was performed the statistical analyses. Results: The comparison between obesity and control groups respectively by the mean of the anthropometric and biochemical parameters levels that showed a significantly increased in BMI (30±2 versus 21±1, P=0.04), W/H (1.5±0.09 versus 0.82±0.07, P=0.02), TNF-α (11±0.8 versus 4±0.3, P=0.04), FSH (12±1 versus 10±0.5, P=0.04) and FN1 (400±25 versus 300±20, P=0.03). FN1 showed a significant strong positive correlation with BMI; W/H; TNF-α; and FSH levels in obesity patients. Conclusions: A significant strong positive association of FN1 levels with BMI, W/H, TNF-α and FSH in patients with obesity. Therefore, FN1 levels may be used as an early diagnostic marker to identify infertility in obesity patients.