Background and Aim: In stimulants dependence, the initial immune defense that play a role in response to central nervous system (CNS) inflammation. A novel nucleotide (TRB3 Q84) has been linked to a genetic variant with several metabolic disorders. In serum of individuals with stimulants dependence, assess of TRB3 Q84 levels and find associations with other markers, this was our aim in this study. Results: The *mean* of TRB3 Q84, IL-6 and CTX levels showed a significantly increased in stimulants dependence as compared with control. Conclusions: The present study demonstrated that individuals with stimulants dependence exhibited a significantly elevated TRB3 Q84 levels as compared to the group of control. In the turn, TRB3 Q84 had a significant strong direct relationship with (IL-6, CTX) these findings suggest that TRB3 Q84 levels may serve a protective function during the early stages of cellular dysfunction associated with stimulants dependence that contributing to stroke pathogenesis.